The gamma-H2AX Pharmacodynamic Assay Kit is for the study of double strand DNA breaks through the detection of gamma-H2AX in peripheral blood mononuclear cells, cultured cells, and tissue biopsies. Contract Services are also available.

A gamma-H2AX kit and antibody allows the assessment of DNA damage and DNA repair for ELISA based assays, immunohistochemistry or flow cytometry. In the detection of genomic damage, gamma-H2AX is especially important in research involving cancer treatment and …

Däremot detekterades fosforyleringar av ATM och y- H2AX i både 6 Gy uppvisade inte ACOT7 Si-transfekterade celler gamma-H2AX-foci i kärnan (figur 4e). Latexin sensibiliserar leukemogena celler till gamma-bestrålningsinducerad PE-konjugerade antikroppen p-histon H2AX (S139) (Cell Signaling, Danvers, MA I själva verket, strax efter IR, berikades γ- H2AX normalt i foci i Mcl-1 eller IEX-1-bristfälliga celler. Detta överensstämmer med det faktum att IEX-1-expression γ -H2AX: Gamma, H2A histone family, member X. 3′UTR: 3′-Untranslated region. 5-Fu: 5-Fluorouracil. ABCB9: ATP-binding cassette, subfamily B (MDR/TAP) alpha, IFN-gamma-inducible protein 10 (IP-10), IL-lb, his Gamma-aminobutyric acid (GABA) H2AX Foci in Human Lymphocytes from Hypersensitive and. Microwaves from UMTS/GSM mobile phones induce long-lasting inhibition of 53BP1/gamma-H2AX DNA repair foci in human lymphocytes. Bioelectromagnetics.

These results unveil a new interplay between plant H2AX and E2F transcriptional activators during the DNA double-strand breaks response. Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). Phospho-H2AX or γ-H2AX- is a marker of DNA double-stranded breaks and can therefore be used to monitor DNA repair after, for example, irradiation. In addition, positive staining for phospho-H2AX may indicate genomic instability and telomere dysfunction in tumour cells and tissues. The results suggest gamma-H2AX is a useful adjunct in diagnosis of metastatic RCC when RCC-Ma is negative and in higher grade RCC, which are often a diagnostic challenge. A nuclear pattern of staining of gamma-H2AX has a comparable sensitivity with RCC-Ma, and the interpretation is easier and more reliable.

Hittade 3 avhandlingar innehållade ordet gamma-H2AX. 1.

Gamma-H2AX foci in cells exposed to a mixed beam of X-rays and alpha particles Elina Staaf1*, Karl Brehwens1, Siamak Haghdoost1, Joanna Czub2 and Andrzej Wojcik1,3 Abstract Background: Little is known about the cellular effects of exposure to mixed beams of …

Se hela listan på academic.oup.com 2017-02-03 · Phosphorylated H2AX (γ-H2AX) is a sensitive marker for DNA double-strand breaks (DSBs), but the variability of H2AX expression in different cell and tissue types makes it difficult to interpret the meaning of the γ-H2AX level. Furthermore, the assays commonly used for γ-H2AX detection utilize laborious and low-throughput microscopy-based methods. We describe here an ELISA assay that Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle.

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Regarding In situ detection of DNA double strand breaks by immunofluorescent gamma-H2AX staining in mice exposed to multiwalled carbon nanotubes. Aimonen, K. The nucleotide pool, a target for low-dose γ-ray-induced oxidative stress Gamma-H2AX foci in cells exposed to a mixed beam of X-rays and alpha particles.

PMID 16310392 : Tyrosine dephosphorylation of H2AX modulates apoptosis and survival decisions.
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Gamma-H2AX can also be measured by immunohistochemistry.

GCDH Medarbetarens roll/uppgift: MedDr Yue Gao kommer att utföra P-H2AX analyserna An optimized method for detecting gamma-H2AX in blood cells reveals a Igor Belyaev m.fl. - Microwaves from UMTS/GSM mobile phones induce long-lasting inhibition of 53BP1/gamma-H2AX DNA repair foci in human lymphocytes. DNA damage (double-strand break; formation of gamma-H2AX foci (fluorescence microscopy) and phosphorylation of gamma-H2AX (Western blot)) and DNA Microwaves from GSM mobile telephones affect 53BP1 and [gamma]-H2AX foci in human lymphocytes from hypersensitive and healthy persons( ) 1 edition H2AX and Western Blot (Lab E224).
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Total lysates (15 µg protein) from untreated HeLa (Lane 1) and UV treated HeLa (Lane 2) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.1 µg/mL Purified anti-H2A.X Phospho (Ser139) antibody, clone 2F3 (upper) or 1:2000 diluted anti-GAPDH antibody, clone 1D4 (lower).

Phosphorylation of histone H2AX to form γ-H2AX is a known marker for irradiation-induced DNA DSBs. However, the first generation assay involves the use of immunofluorescent H2AFX (famille des histones H2A, membre X) est l'un des plusieurs gènes codant l'histone H2A.Chez l'homme et les autres eucaryotes, l'ADN est enroulé autour de groupes histones, comprenant les histones de cœur H2A, H2B, H3 et H4. Se hela listan på hindawi.com gamma H2AX and cleaved PARP-1 as apoptotic markers in irradiated breast cancer BT474 cellular spheroids Qvarnström, O. F (author) Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi Simonsson, M (author) Uppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi Eriksson, V (author) 2021-02-25 · p-Histone H2A.X Antibody (Ser 139) is a mouse monoclonal IgG 1, cited in 38 publications, provided at 100 µg/ml; raised against a recombinant protein corresponding to the Ser 139 phosphorylated region of Histone H2A.X of human origin Anti-phospho-H2A.X (Ser139) Antibody Upstate®, from rabbit; Synonym: H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac; find Sigma-Aldrich-07-164 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich.

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2016-03-31

För dig som är. Medarbetare Patient Vårdgivare Vårdhygien Regional laboratoriemedicin Analyslistor, provtagningsanvisningar Gamma H2AX (gammaH2AX) is the phosphorylated version of histone H2AX and is a marker for double-stranded breaks (DSBs) caused by DNA damage (1-4). H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4).